Quantifying AAV empty/full ratios with mass photometry

Quantification of empty/full ratio of capsids in AAV preparations is a major bottleneck in characterizing recombinant AAV samples. A novel, fast and easy method to determine these ratios is mass photometry, which is a single-particle technique that measures the masses of AAVs in solution with minimal sample preparation. SamuxMP is a mass photometer, tailored specifically to measure empty/full ratios of AAVs. In this talk, we will demonstrate capabilities of the SamuxMP in characterizing AAV samples. We will show that it can differentiate between empty, partially filled and full AAV capsids, and that is serotype agnostic. We will also show data demonstrating that the SamuxMP measurements are in line with gold-standard techniques such as TEM and AUC.